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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Hepatology. 2017 Jul 20;66(3):869–884. doi: 10.1002/hep.29145

Figure 6. The role of H19 in TCA-/E2-mediated expression of pro-fibrotic genes in cholangiocytes.

Figure 6

MLE cells were transduced with control or H19 shRNA adenovirus for 6h before treatment with a vehicle control, TCA (100 μM), E2 (100 nM) or TCA+E2 for 48h. The relative expression levels of H19 (A) and mRNA levels of α-SMA (B), Bcl-2 (C), collagen 1 (D), SHP (E) and S1PR2 (F) were determined by real-time RT-PCR and normalized using HPRT1 as an internal control. Statistical significance: *P<0.05, **P<0.01, compared with vehicle control group; #P<0.05, compared with C-shRNA E2 group; $P<0.05, $$P<0.01, compared with C-shRNA TCA group; ΦP<0.05, ΦΦP<0.01, compared with C-shRNA TCA+E2 group (n=3).