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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2017 Jul 27;37(9):1748–1756. doi: 10.1161/ATVBAHA.117.309735

Fig. 4. Expression of ADAMTS13 in primary placental trophoblasts and HTR-8/SVNEO cells.

Fig. 4

A. Relative ratios of ADAMTS13 to GAPDH mRNA levels in placental trophoblasts. Human liver ADAMTS13 mRNA was used as a positive control and blank CHO cell mRNA was used as a negative control. B. Western blotting demonstrates a full-length ADAMTS13 protein in the cell lysate (Lys) and conditioned medium (Med) of primary trophoblast (TB) and HTR-8/SVNEO cells. Recombiannt ADAMTS13 (rA13) expressed in CHO cells transiently transfected with ADAMTS13 plasmid was used as a positive control. C. ADAMTS13 activity (mean ± SEM) in the conditioned media of primary trophoblasts (TB) and HTR-8/SVNEO cells is shown. D. Immunofluoreceent staining of primary trophoblasts (TB) and HTR-8/SVNEO cells with monoclonal anti-CK7 (10μg/ml) and rabbit anti-ADAMTS13 IgG (20μg/ml) as indicated in the photographs (Bar=50μm). CK7 is a marker for placental trophoblasts.