(A) Survival curve following collagen/epinephrine induced pulmonary embolism, n=22. (B) Washed platelets were incubated on collagen or fibrinogen coated chamber slides under static conditions in the presence or absence of 0.1U/mL thrombin (IIa), and stained with phalloidin (green) for cytoskeletal changes, scale 5μm, n=4. (C) Spreading was quantified as summarized in the bar graphs, n=4. (D) Representative image of clot retraction assay performed in diluted whole blood stimulated with thrombin and data quantification (E), n=4. Data are mean±SEM. *P<0.05, **P<0.01 vs. control same treatment; 2-way ANOVA followed by Tukey’s multiple comparison post hoc test (E); Student’s t test (C); Log-rank (Mantel-Cox) test (A.).