Table 2.
PCR and primers used in this study
| Primer ID | Sequence (5′ > 3′) | PCR ID | Product length (bp) | Organism | References |
|---|---|---|---|---|---|
| 1803-f | TTT GGA ACA ATG GAT GCT GA | 748 | 746 | Murine NoV (first cycle) | This study |
| 1804-r | AGT CGA CCA TCC GGT AGA TG | ||||
| 1801-f | AAT TGA CCC CTG GAT CTT CC | 747 | 352 | Murine NoV (nested cycle) | |
| 1802-r | GAC TCG ACG CAC ATC AAG AA | ||||
| 1798-f | GGC TGG GTT GGG AAC ATG | 746 | 84 | Murine NoV (real-time) | This study |
| 1799-r | TGG TAC AAG GGC AAC AAC CA | ||||
| 1800-f | CTG GTC CTC GCC GGC AAT GC (5′FAM 3′TAMRA) | ||||
| 1531-f | GCA CTC GGC ATC ATG ACA AAA TTC A | 612 | 995 | GIV NoV (first cycle) | La Rosa et al. (2008) |
| 1532-r | GTT TGG GTC CCA ATT CCA A | ||||
| 1698-f | GTA CTG GAC CAA GGG CCC GA | 686 | 323 | GIV NoV (nested cycle) | This study |
| 1699-r | GAG GTT GCC CGC ACC ATC CG | ||||
| 1773-f (MON 4F) | TTT GAG TCY ATG TAC AAG TGG ATG C | 756 | 133 | GIV NoV (real-time) | Trujillo et al. (2006), this study |
| 1790a-r | GTT GCC CGC ACC ATC CGY AG | ||||
| 1775-f (RING 4) | TGG GAG GGG GAT CGC GAT CT (5′FAM-3′BHQ) |
a
Replaces the original MON4R (Trujillo et al. 2006)