Detailed Definitions for Sociodemographics, Health Behaviors, Chronic Medical Conditions, and Biomarkers

Variable	Definition
Sociodemographics
Age	Age in years, dichotomized to ≥75 years vs <75 years
Gender	Male, female
Race	African American, white
Education	Participant reported:
	Less than high school
	High school graduate
	Some college
	College or higher
	Missing
Income	Participant reported:
	<$20k
	$20k to $34k
	$35k to $74k
	≥$75k
	Missing (not reported)
Geographic region	Participant residence:
	Stroke buckle (coastal plains of North Carolina, South Carolina, and Georgia)
	Stroke belt (remainder of North Carolina, South Carolina and Georgia, plus Tennessee, Mississippi, Alabama, Louisiana, and Arkansas)
	Non-belt/buckle (other states)
Health behaviors
Smoking status	Participant reported:
	Current
	Past
	Never
Alcohol use	Participant reported:
	None
	Moderate (up to 1 drink per day for women or 2 drinks per day for men)
	Heavy (>1 drink per day for women and >2 drinks per day for men)39
Chronic medical conditions
Atrial fibrillation	Participant reported history of atrial fibrillation
Chronic lung disease	Participant use of pulmonary medications (β agonists, leukotriene inhibitors, inhaled corticosteroids, combination inhalers, ipratropium, cromolyn, aminophylline, and theophylline) as a surrogate for chronic lung disease
Coronary artery disease	Participant reported history of myocardial infarction, coronary artery bypass grafting, or cardiac angioplasty or stenting, or baseline electrocardiographic evidence of myocardial infarction
Diabetes	Fasting glucose ≥ 126 mg/L (or a glucose ≥200 mg/L for those not fasting) or participant-reported use of insulin or oral hypoglycemic agents
Deep vein thrombosis	Participant reported history of deep vein thrombosis
Dyslipidemia	Low-density lipoprotein cholesterol >130 mg/dL or participant reported usage of lipid-lowering medications
Hypertension	Systolic blood pressure ≥ 140 mm Hg, diastolic blood pressure ≥90 mm Hg, or participant reported antihypertensive agent usage
Obesity	{Waist circumference [>102 cm for males or >88 cm for females]} or {body mass index ≥30 kg/m}.
Peripheral artery disease	Participant reported history of lower extremity arterial bypass or leg amputation
Stroke	Participant reported history of stroke or transient ischemic attack
Biomarkers
Estimated glomerular filtration rate (eGFR)	Abnormal defined as eGFR <60 mL/min/1.73m2
	Assay by colorimetric reflectance spectrophotometry (Ortho Vitros Clinical Chemistry System 950IRC, Johnson & Johnson Clinical Diagnostics, Raritan, New Jersey). eGFR based upon CKD-Epi equation40
Albumin-to-creatinine ratio (ACR)	Abnormal defined as ACR ≥30 mcg/mg
	Albumin assay by nephelometry (BN ProSpec Nephelometer, Dade Behring, Siemens Healthcare, Deerfield, Illinois). Urinary creatinine assay determined by rate blanked Jaffe´ procedure (Modular-P analyzer, Roche/ Hitachi, Roche Diagnostics, Indianapolis, Indiana).
High-sensitivity C-reactive protein (hsCRP)	Abnormal defined as hsCRP >3.0 mg/dL
	Assay by particle-enhanced immunonephelometry (N High-sensitivity CRP, Siemens AG, Munich, Germany).