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Enhancement of PCR amplification of c-jun (996 bp segment) cDNA by 2-pyrrolidone, formamide and acetamide. Lane 1, 0.4 M 2-pyrrolidone; lane 2, 0.8 M formamide; lane 3, 0.8 M acetamide; lane 4, control (no additive); lane 5, 100 bp DNA ladder (Gibco).
