Table 5.
Relative efficiency of molecular markers for determining polymorphism in Citrullus colocynthis
| S. no. | Parameters for marker efficiency | Molecular marker system | ||
|---|---|---|---|---|
| RAPD | ISSR | RAPD + ISSR | ||
| 1. | Number of individuals | 38 | 38 | 38 |
| 2. | Total number of scorable bands (NSB = L) | 549 | 166 | 715 |
| 3. | Total no. of assays/primers combination (T) | 50 | 13 | 63 |
| 4. | Total number of Polymorphic bands (p) | 318 | 99 | 417 |
| 5. | Multiplex ratio (MR) = (L/T) | 10.98 | 12.76 | 11.34 |
| 6. | % Polymorphism | 55.14 | 65.04 | 60.09 |
| 7. | Average polymorphism (bands primer−1) | 6.36 | 7.60 | 6.98 |
| 8. | PIC average (PICavg) | 0.47 | 0.44 | 0.46 |
| 9. | Average heterozygosity (H avg) | 0.49 | 0.46 | 0.48 |
| 10. | Marker index (MI) = H avg × MR | 5.32 | 5.86 | 5.45 |
| 11. | Average informativeness of a band (BIi) | 0.45 | 0.46 | 0.45 |
| 12. | Resolving power (RP) | 2.98 | 4.10 | 3.21 |