Table 1.
Operating conditions and accessories employed in ICP-OES spectrometer (Shimadzu ICPE-9820) for analysis of intervertebral discs samples
| Parameter and accessories | Value | |
|---|---|---|
| Radio frequency power generator | 1.2 kW | |
| Gas type | Argon | |
| Plasma gas flow rate | 10.0 L min−1 | |
| Auxiliary gas flow rate | 0.6 L min−1 | |
| Nebulization gas flow rate | 0.7 L min−1 | |
| Plasma view | Vertical torch; axial view | |
| Selected emission lines (atomic) | 251.611 (BEC = 0.084) | |
| 212.412 (BEC = 0.188) | ||
| Torch | Mini-torch (quartz) | |
| Nebulizer | Burgener NX-175 | |
| Chamber | Cyclone (glass) | |
| Drain | Gravity fed | |
| Injector tube | Quartz (1.2 mm i.d.) | |
| Background correction | 2-points | |
| Number of replicates | 3 | |
| Exposure time | 15 s | |
| Peristaltic pump | Solvent rinse | 15 s |
| Sample rinse | 15 s | |
| Sample uptake rate | 1 ml min−1 | |
| Spectrometer | Echelle optics | Range of wavelength:167 to 800 nm |
| Resolution | ≤0.005 nm at 200 nm | |
| Atmospheric removal system | Rotary vacuum pump ≤10 Pa | |
| Device | CCD (charge coupled device) detector | |
| Pixel number | 1024 × 1024 pixels (1 in.) | |
| Pixel size | 20 μm × 20 μm | |
| Cooling control | Peltier device | |