. 2017 Aug 24;7:9397. doi: 10.1038/s41598-017-09645-9

Table 2.

EC₅₀ values shown for the Cl⁻/NO₃ ⁻ antiport assay, the NMDG-Cl assay alone, with gramicidin (Gra) and with oleic acid (OA), with the chloride selectivity (S_G) and the factor of enhancement (F_OA) included.

Perenosin	clog P^a	Cl⁻/NO₃ ⁻ at pH 7.2 (by ISE)		NMDG-Cl (by HPTS)
Perenosin	clog P^a	EC₅₀ (mol%)^b	n^c	EC₅₀ (mol%)^d	EC_{50 (Gra)} (mol%)^e	EC_{50 (OA)} (mol%)^f	S_G ^g	F_OA ^h
1d	5.1	0.1220	1.2	0.01073	0.00656	0.00925	1.6	1.2
2	4.9	0.0043	1.3	0.00062	0.00037	0.00085	1.7	0.7
3	5.7	n.d.ⁱ	n.d.ⁱ	0.01151	0.00879	0.04912	1.3	0.2
4	4.4	0.0111	1.1	0.00069	0.00038	0.00008	1.8	8.6
5	5.3	0.0227	1.3	0.00231	0.00095	0.00273	2.4	0.9
6	6.9	0.0852	0.3	0.00249	0.00211	0.00208	1.2	1.2
7	6.1	>10^j	n/a^j	n.d.^j	n.d.^j	n.d.^j	n/a^j	n/a^j
8	6.2	>10^j	n/a^j	n.d.^j	n.d.^j	n.d.^j	n/a^j	n/a^j

^aaverage clog P values calculated using VCCLab⁶². ^bEC₅₀ at 270 s, shown as carrier: lipid molar percent. ^cHill coefficient reveals the stoichiometry of the complex mediating transport. ^dEC₅₀ from the NMDG-Cl assay, measuring H⁺/Cl⁻ symport. ^eEC₅₀ in the presence of Gra, showing the total H⁺/Cl⁻ symport possible with no rate limiting H⁺/OH⁻ transport. Gra concentration has been optimised to 0.1 mol%. ^fEC₅₀ in the presence of OA, showing changes in transport due to acceleration of the natural proton transport facilitated by fatty acids. OA concentration has been optimised to 2 mol%. ^gS_G > 1 indicates Cl⁻ selectivity and is calculated by dividing EC₅₀ in absence of Gra by EC_{50 (Gra)}. ^hFactor of enhancement in the overall H⁺/Cl⁻ cotransport rate is calculated by dividing EC₅₀ in absence of Gra or OA by EC_{50 (OA)}. F_OA > 1 suggests the receptor can assist the flip-flop of OA⁻ increasing pH dissipation. ⁱn.d. EC₅₀ not determined by ISE due to poor solubility. ^jWeak transport ability where EC₅₀ for Cl⁻/NO₃ ⁻ by ISE is larger than 10 mol%, hence NMDG-Cl by HPTS assay not determined (n.d.).