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. 2017 Aug 24;7:9319. doi: 10.1038/s41598-017-07188-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The mlo2 mlo6 mlo12 triple mutants show an unaltered A. laibachii infection phenotype. Six-week-old plants were spray-inoculated with A. laibachii (isolate Nc14) and infection phenotypes were assessed at 10 dpi. (A) Representative examples of inoculated rosette leaves of accessions Col-0, Ksk-1 and the two mlo2 mlo6 mlo12 triple mutant lines. Insets show a magnification of an inoculated leaf area. (B) Quantitative evaluation of the infection rate. Data show the mean ± standard deviation (SD) of the proportion of rosette leaves with disease symptoms (macroscopically visible white rust pustule formation) based on 5-6 plants per genotype and 12-30 evaluated leaves per plant. ** Indicates a statistically significant difference from Col-0 (P < 0.01) according to a Wilcoxon-Mann-Whitney rank sum test.