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. 2017 Aug 24;7:9345. doi: 10.1038/s41598-017-09764-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expressions of HDAC1 and DNMT1 by EGCG and/or SFN treatment. Quantitative real-time PCR was performed to measure relative transcription of HDAC1 (A) and DNMT1 (B) in MDA-MB-231 cells and MDA-MB-157 cells. (C) Protein expression of HDAC1 and DNMT1 in MDA-MB-231 and MDA-MB-157 cells. β-actin was loaded as an internal control. The full-length blots were shown in the Supplementary Information. (D) HDAC1 protein quantification. (E) DNMT1 protein quantification. Data were in triplicate from three independent experiments and normalized to internal control and calibrated to levels in untreated samples. E + S, EGCG and SFN in combination; Columns, mean; Bars, SD; *p < 0.05; **p < 0.01; ***p < 0.001, significantly different from control or the indicated comparisons.