Skip to main content
. 2017 Aug 24;7:9378. doi: 10.1038/s41598-017-09933-4

Figure 6.

Figure 6

Effects of delphinidin on proliferation and Ca2+ signaling of T lymphocytes from non-MetS and MetS patients. (A) Histograms show the percentage of proliferation of cells isolated from non-MetS patients exposed to either 10−2 g/L of delphinidin (Del), 5 µg/mL PHA or both for 24 and 48 h. (B) Same experiments on cells isolated from MetS patients. Data are the mean ± SEM (n = 8–31). (C) Representative traces (left) and histograms (right) showing the mean of the responses induced by 10−2 g/L of delphinidin (Del) alone or after activation by 1 µM thapsigargin (TG) on [Ca2+]i in Ca2+-containing PSS of T cells isolated from non-MetS patients. (D) Representative traces (left) and histogram (right) showing the effect of 10−2 g/L Del on [Ca2+]i increase induced by 1.25 mM of CaCl2 after depletion of intracellular stores in Ca2+-free PSS by 1 µM TG of cells isolated from non-MetS patients. Data are the mean ± SEM (n = 12–14). (E and F) Same experiments as (C and D, respectively) on cells isolated from MetS patients. (G) Negative correlation between Del effect on PHA-induced proliferation and number of MetS criteria. Data are the mean ± SEM (n = 16). *P < 0.05, **P < 0.01 and ***P < 0.001. ***P < 0.001 versus control group, # P < 0.05 versus TG group.