Fig. 4.

PIF5 directly represses the expression of MIR156 genes. a Transient expression assays shows that PIF5 directly represses the expression of MIR156B, MIR156D, MIR156E, MIR156F, and MIR156H. Representative images of N. benthamiana leaves 72 h after infiltration were shown. b Quantitative analysis of luminescence intensity in a. Five independent determinations were assessed. Values shown are mean ± SD (n = 5). **P < 0.01 by the Student’s t-test. c Dual-luciferase assay of pMIR156::LUC expression. The expression of REN was used as an internal control. LUC/REN ration represents the relative activity of the MIR156 promoter. Values given are mean ± SD (n = 3). **P < 0.01 by the Student’s t-test. d Transcript levels of mature MIR156 analyzed by qRT-PCR. Eight-day-old WT, PIF overexpressors, and pifq seedlings grown under WL were harvested for RNA extraction. Values given are mean ± SD (n = 3). *P < 0.05 and **P < 0.01 by the Student’s t-test. e The relative expression levels of individual primary MIR156 in 8-day-old WT, PIF overexpression, and pifq seedlings grown under high R:FR conditions. Values given are mean ± SD (n = 3). *P < 0.05 and **P < 0.01 by the Student’s t-test