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. 2017 Aug 24;7:9327. doi: 10.1038/s41598-017-09297-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) MtBRI1 protein structure showing leucine-rich domains (LRR) and the kinase domain analysed in the Expysa-Prosite website. Represents the signal peptide; represents the transmembrane domain. (b) Phylogenetic tree of MtBRI1and other known BRI proteins analysed using the MegAlign software (DNAstar). LKA, pea, AB104529.1; GmBRI1: soybean, FJ014768.1; BRI1: Arabidopsis, NM120100.2; BnBRI1: Brasssica napus, JX868513.1; NbBRI1: Nicotiana benthamiana, EF471738.1; SpBRI1: Solanum pimpinellifolium, EF471736.1; TaBRI1: Triticum aestivum, DQ655711.1; HvBRI1: Hordeum vulgare, AB088206.1; OsBRI1: Oryza sativa (Japonica), NM_001050612.1. (c) MtBRI expression patterns from the Medicago Gene Expression Atlas, which is represent by probe set Mtr.45830.1.S1_at.

Inline graphic — (a) MtBRI1 protein structure showing leucine-rich domains (LRR) and the kinase domain analysed in the Expysa-Prosite website. Represents the signal peptide; represents the transmembrane domain. (b) Phylogenetic tree of MtBRI1and other known BRI proteins analysed using the MegAlign software (DNAstar). LKA, pea, AB104529.1; GmBRI1: soybean, FJ014768.1; BRI1: Arabidopsis, NM120100.2; BnBRI1: Brasssica napus, JX868513.1; NbBRI1: Nicotiana benthamiana, EF471738.1; SpBRI1: Solanum pimpinellifolium, EF471736.1; TaBRI1: Triticum aestivum, DQ655711.1; HvBRI1: Hordeum vulgare, AB088206.1; OsBRI1: Oryza sativa (Japonica), NM_001050612.1. (c) MtBRI expression patterns from the Medicago Gene Expression Atlas, which is represent by probe set Mtr.45830.1.S1_at.