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. 2017 Aug 24;7:9327. doi: 10.1038/s41598-017-09297-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypes of mtbri1 mutants. (a–c) 8-day-old seedlings grown on ½ MS media under light. (a) mtbri1-1 & wild type (WT); (b) mtbri1-2 & WT; (c) mtbri1-3 & WT. (d) Comparison of 8-day-old seedlings between wild type and mutant seedlings grown under light or dark. (e) One-month-old mtbri1-1 and wild type plants grown in soil. (f,g) Two-month-old mtbri1-1 and wild type plants grown in soil. (h) Wild type R108 plants at 21 days post-inoculation with nodules shown in the inset. (i) mtbri1-1 plants at 21 days post-inoculation with nodules shown in the inset. (j) Nodule numbers per plant in wild type R108 and mtbri1-1. Error bars represent the standard error of the means (n ≥ 10). Means with different letters are significantly different (P < 0.005; Tukey’s test). Scale bars: 10 mm.