Figure 7.

BIRC3 silencing impairs hypoxia-induced survival of GBM to radiotherapy (RT). 1 × 10⁴ U87 MG GBM cells were cultured in 96 well plate under hypoxia (1% O₂) condition for 12 hr and irradiated with 4 Gy. Cells were returned to hypoxia conditions for another 12 hr and harvested. BIRC3 mRNA and protein expression were analyzed by RT-PCR (A) and Western blot, respectively (B). Similar results were obtained from three independent experiments (p < 0.05). Whole images for Western-blot can be found in the Supplementary Figure 9. (C) U87 GBM cells with or without BIRC3 siRNA pretreatment (48 hr earlier) were cultured under hypoxia (1% O₂) for 12 hr and irradiated with 2 Gy, 4 Gy, 6 Gy or 8 Gy. Cells were returned to hypoxia conditions for another 24 hr and cell survival were assessed using an XTT Cell Viability Assay Kit. Data are representative of three independent experiments (p < 0.05).