Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 24;8:344. doi: 10.1038/s41467-017-00324-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — LCL161 therapy rescues CD8⁺ T-cell exhaustion within the TME. a Intracellular staining for IFNγ or TNFα within CD8⁺ T cells isolated from EMT6 tumors 7 days post treatment and stimulated with PMA and ionomycin ex vivo, measured by flow cytometry (duplicate experiments; mean ± SD; ANOVA with Bonferroni’s multiple comparisons test). b Membrane staining for PD-1 and Tim-3 expression on CD8⁺ T cells isolated from 12-day-old EMT6 tumors, measured by flow cytometry (duplicate experiments; mean ± SD). c CD8⁺ T cells within EMT6 tumors 7 days post treatment, measured by flow cytometry (triplicate experiments; mean ± SD; t-test). d Intracellular staining for IFNγ or TNFα within CD8⁺ T cells isolated from EMT6 TdLN 7 days post treatment and stimulated with PMA and ionomycin, measured by flow cytometry (single experiment; mean ± SD; ANOVA with Bonferroni’s multiple comparisons test). e Membrane staining for PD-1 and Tim-3 expression on CD8⁺ T cells isolated from 12-day-old EMT6 TdLNs, measured by flow cytometry (duplicate experiment; mean ± SD). f Activation of CD8⁺ T cells isolated from TdLN 8 days post treatment, after co-culture with EMT6 cells, measured by IFNγ ELISpot assay (n = 3 biological replicates, single experiment; mean ± SD; ANOVA with Tukey’s multiple comparisons test). g CD8⁺ T cells within EMT6 TdLN 7 days post treatment, measured by flow cytometry (duplicate experiments; mean ± SD; t-test). h Intracellular staining for IFNγ or TNFα within CD8⁺ T cells isolated from 12-day-old EMT6 tumors and treated in vitro with LCL161 at the indicated concentrations for 24 h, measured by flow cytometry (n = 2 biological replicates, single experiment; mean ± SD; ANOVA with Bonferroni’s multiple comparisons test)