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. 2017 Aug 24;7:9399. doi: 10.1038/s41598-017-09978-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Polymorphisms of ATG5 enhanced the inflammatory cytokines release of human monocytes. PBMCs isolated from 55 healthy volunteers carrying different genotypes of ATG5 rs510432/rs506027 polymorphisms were incubated in RPMI1640 (10% fetal calf serum) and stimulated with 500 ng/mL LPS for 8 h. TNF-α, IL-6 and IL-1β were measured in the supernatants by ELISA (A–F). The horizontal line indicates the mean levels with each genotype groups. *p < 0.05; **p < 0.01; ***p < 0.001.