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. 2017 Aug 24;7:9285. doi: 10.1038/s41598-017-08249-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DACT1 expression increased 3AO chemoresponse to cis-platinum by enhancing autophagy. (a) and (b) Viability of ovarian cancer cell lines incubated with cis-platinum (CDDP), measured by CCK-8 assay. (****P < 0.0001). (c) and (d) Viability of 3AO-DACT1 or 3AO-NC cells incubated with the indicated concentrations of cis-platinum (****P < 0.0001, paired t test). (e) and (f) Accumulation of markers of autophagy in 3AO-DACT1 or 3AO-NC cells was assessed by Western blot. Increased levels of Beclin1 and the lipidated form of LC3 (LC3-II/LC3-I) were detected in 3AO-DACT1 cells. α-tubulin was used as the loading control. Grey values collected by Fusion were analysed (*P < 0.05, **P < 0.01). (g) and (h) Viability of 3AO-DACT1 or 3AO-NC cells incubated with the indicated concentrations of cis-platinum in the presence or absence of chloroquine (***P < 0.001, paired t test, P < 0.05, **P < 0.001, ****P < 0.0001). All the experiment was repeated at least three times.