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. 2017 Aug 24;7:9341. doi: 10.1038/s41598-017-10022-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of transcript levels of stress responsive marker genes in activation tagged DS-16 line in comparison with WT-BPT under NaCl stress treatment. (A) OsP5CS1; (B) OsNHX1; (C) OsSOS1; (D) OsNAC6; (E) OsbZIP23; (F) OsLEA3; (G) OsSalT. Two weeks old seedlings grown in Yoshida’s solution supplemented with 150 mM NaCl for 15 d and those without NaCl supplement used as control. OsActin1 was used as a reference gene for quantitative real-time RT-PCR. Means of three independent samples and standard errors are presented. ** and * indicates significant difference at P < 0.01 and P < 0.05.