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. 2017 Aug 24;7:9326. doi: 10.1038/s41598-017-04307-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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INCh1 characterisation using a microarray populated with starch-related samples. (a) Heatmap showing INCh1 binding to a carbohydrate microarray populated with starch-related oligo- and polysaccharides and enzymatic epitope deletion. Note that INCh1 bound to α-(1-4)-glucan oligomers with a minimum degree of polymerisation of 4. INCh1 binding was abolished by pre-treatment of arrays with amyloglucosidase but not by isoamylase, pullulanase or any non-starch related control enzymes. Colour intensity in the heatmap is correlated to mean spot signals. The highest signal in each dataset was set to 100, and all other values were normalized accordingly as indicated by the colour scale bar. A low-end cut-off value of 5 was imposed. (b) Diagram showing the cleavage sites of the starch degrading enzymes used.