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. 2017 Aug 24;7:9326. doi: 10.1038/s41598-017-04307-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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INCh1 and ECG1A9 mAb labelling of starch granules in pea root cap. Images showing immunofluorescence labelling of starch granules by INCh1 (a) and ECG1A9 (b) in resin-imbedded sections through pea root caps. (c) No primary antibody control. Images shown in (a–c) are overlays of signals from three channels: Calcofluor White (staining cellulose, blue), propidium iodide (staining nuclei, red) and immunolabeling using an anti-mouse conjugated Alexa Fluor 488 as secondary antibody (green). The lower panels show differential interference contrast images obtained from the same sections. Starch granules are marked with arrowheads. Scale bars = 10 μm.