Figure 5.

Simultaneous binding requires both RRMs. Mixtures containing [³²P]-hTR-A, GST, recombinant UP1 or derivatives (UP1Δ1 and UP1Δ2) were loaded onto TS10 columns. Top panels are acrylamide–urea gels used to fractionate ³²P-labeled hTR-A. Bottom panels are silver-stained protein gels. I, input; FT, flow-through fraction; W, wash (a pool of three washes with buffer DN containing 150 mM KCl). Half of the I, F, W and eluted fractions were loaded on the RNA gel and the other half on a protein gel. The positions of the proteins and hTR-A are indicated.