Figure 6.

The TS10/A1/hTR-A ternary complex resists incubation in a nuclear extract. Left, hTR-A and recombinant A1 were mixed and loaded onto the TS10 column. Middle, hTR-A and recombinant A1 were incubated together and mixed in a HeLa S100 extract. The mixture was then loaded onto a TS10 column. Right, as in the middle panel except that A1 was omitted. In all cases, RNA recovered from each fraction was fractionated on a denaturing polyacrylamide gel. I, input (total fraction); FT, flow-through fraction (total fraction); W, wash fractions (total fractions). Elutions 1, 2, 3 and 4 are successive elutions with buffer DN containing 250, 500 or 750 mM or 1 M KCl, respectively. The position of hTR-A is indicated.