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. 2001 Jun 1;29(11):2284–2291. doi: 10.1093/nar/29.11.2284

Figure 1.

Figure 1

Figure 1

Figure 1

CSL activates transcription in 293T cells. (A) Activity of a CSL-responsive reporter, (CSL)4-luc, compared to the reporter lacking CSL binding sites, pGl2pro, upon transfection of NIH 3T3 and 293T cells. (B) EMSAs using nuclear extracts of NIH 3T3 and 293T cells in conjunction with an oligonucleotide bearing CSL binding sites. The major band is due to CSL by virtue of the change of its mobility in the presence of CSL antiserum (+ αCSL), but not in the presence of control serum (+ Serum). (C) Activity of a Gal4-CSL fusion protein transfected into NIH 3T3 and 293T cells. Cells were transfected with either the Gal4 site-containing reporter alone (5×Gal4-TK-luc) or along with an expression plasmid expressing Gal4-CSL, as indicated. Luciferase activities were determined 2 days after transfection.