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. 2017 Aug 24;7:9373. doi: 10.1038/s41598-017-09668-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Combination of NCTD and CLSO significantly inhibits cell viability in HepG2 and HepG2/ADM cells. (a) The percentage of viable cells as measured by the MTT assay at 24 and 48 h relative to no-drug controls and NCTD concentrations were plotted as a dose response curve (n = 6 per group). (b) Cells treated with the CLSO(n = 6 per group). (c) Cells treated with the combination of NCTD and CLSO. Cell viability was assayed (n = 6 per group). (d) Cytotoxicity of bare microemulsion formulation (containing Span-80 1 g and Sween-80 1.5 g) against HepG2 and HepG2/ADM cells. ^*P < 0.05; ^**P < 0.01, vs. control, ^aP < 0.05; ^aaP < 0.01 vs. NCTD alone, ^bP < 0.05; ^bbP < 0.01 vs. CLSO alone. One-way ANOVA, post hoc comparisons, Tukey’s test. Columns, means; error bars, SDs.