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. 2017 Aug 24;7:9301. doi: 10.1038/s41598-017-08068-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nucleotide and amino acid differences between SA11 NSP2 and AU-1 NSP2 in the 482–1000 region. (a) NSP2 genes of recombinant reassortant viruses with wildtype SA11 or chimeric NSP2 genes are shown as boxes (not drawn to scale). For all genes, SA11 sequence is shown in grey and AU-1 sequence is shown in orange. Nucleotide numbers are listed at the top and the general locations of untranslated regions (UTRs) and open-reading frame (ORF) in the NSP2 gene are shown. The results of the rescue experiments, including % recombinant plaques, are listed to the right of each gene. (b) The sequence of nucleotides 482–1000 of SA11 NSP2 are shown in grey. The nucleotides that differ for AU-1 NSP2 are shown below the SA11 sequence and are colored orange. Amino acid changes are listed beneath the corresponding codon with the SA11 residue shown in grey and the AU-1 residue shown in orange. The SR (nucleotides 784–820) is outlined with a box and labeled.