Figure 7.

Morphological and intracellular changes of RBCs over time when melittin solutions of 50 nM (blue lines), 250 nM (green lines), 1 μM (black lines), and 5 μM (red lines) were added: (A) cell volumes, (B) surface areas, (C) sphericities, (D) Hb concentrations, and (E) dry masses. The bottom x-axis with the black colour is for the time elapsed in one minute for the 50 nM, 250 nM, and 1 μM cases. The top x-axis with the red colour is for the 5 μM case in one second. The red arrows indicate the start of Hb leakage. The grey line in E is the fitted exponential decay.