Table 2.
Acylation status of different CyaA mutants.
| Proteina | K860 | K983 | ||
|---|---|---|---|---|
| Palmitoleyl (%)c | Palmitoyl (%)c | Palmitoleyl (%)c | Palmitoyl (%)c | |
| proCyaAb | 0.2 | 0 | 3.6 | 0.8 |
| CyaA | 44.3 | 45.9 | 55.9 | 22.1 |
| CyaA-Y940F | 38.1 | 57.8 | 41.6 | 20.8 |
| CyaA-Y940A | 39.3 | 55.8 | 56.8 | 22.4 |
| CyaA-Y940P | 42.9 | 50.9 | 55.9 | 19.8 |
aAll CyaA proteins were produced in the E. coli strain XL1-Blue and purified close to homogeneity.
bThe proCyaA variant was expressed in the absence of the CyaC acyltransferase.
cPercentage distribution of fatty acid modification of the ε-amino groups of the K860 and K983 residues by palmitoleyl (cisΔ9 C16:1) and palmitoyl (C16:0) chains. Average values are derived from determinations performed with two different toxin preparations. The remaining Lys860 and Lys983 residues to 100% are non-acylated.