Fig. 1.

Mice with disrupted endothelial Piezo1 are superficially normal. a Physical appearance of control and endothelial Piezo1-deleted (Piezo1^ΔEC) mice. b Body weight and percentage change in body weight of control (n = 13) and Piezo1^ΔEC (n = 14) mice before and 10–14 days after tamoxifen (TAM) treatment. c As percentages of total body weight, weights of heart, lung, kidney and liver in control (n = 13) and Piezo1^ΔEC (n = 14) mice. d Histological examples of control (top row) and Piezo1^ΔEC (bottom row) sections of aorta, heart, lung, kidney and liver stained with H&E. Scale bars 100 μm. e–g Serum concentrations of urea, K⁺ and Na⁺ in control (n = 7) and Piezo1^ΔEC (n = 7) mice. h–m Ultrasound study of the heart h–j and aorta k–m of control (n = 5) and Piezo1^ΔEC (n = 5) mice under anaesthesia. h Example of left ventricle images of control and Piezo1^ΔEC at diastole and systole. The left ventricle chamber is circled with a white dashed line. Scale bar 1 mm. i, j Cardiac parameters measured by ultrasound. k Example of aorta images of control and Piezo1^ΔEC at diastole and systole. The left ventricle chamber is circled with a white dashed line. Scale bar 1 mm. l Aorta anteroposterior diameter at systole. m Aortic distensibility. n Retinal vasculatures stained with isolectin (green) from control and Piezo1^ΔEC mice. Entire retinas (full view) and close up views (zoom). o Quantification of retina distal artery diameter and capillary plexus area from control (n = 5) and Piezo1^ΔEC (n = 4) mice. p Endothelial regeneration 5 days after femoral artery injury. Images of the arteries in which the blue colour shows Evans blue staining of areas which were not re-endothelialized after injury. Scale bars 0.5 mm. q Quantification of endothelial regeneration in control (n = 9) and Piezo1^ΔEC (n = 7) mice. Independent data points are displayed with superimposed bars indicating mean ± s.e.m. Data sets are compared by t-test. No significant differences were detected