FIG 4.
Infection of cell lines stably expressing CD4 variants. (A) Flow cytometric analyses of expression of rhesus CD4, rhesus CD4I39N, or human CD4 on the surfaces of stably transduced Cf2Th-CCR5 cells using an antibody cross-reactive to human and rhesus CD4. HOS.CCR5 cell lines transduced with vector alone are represented in black. Numbers indicate mean fluorescence intensities (MFI). (B) Infection of rhesus CD4 and rhesus CD4I39N cells with luciferase pseudoviruses expressing SIVmac239 or SIVmac239 G382R/H442Y Env and normalized to infection observed with human CD4. (C) An experiment similar to that in panel A except that HOS.CCR5 cells stably expressing CD4 variants were analyzed. (D) An infection experiment similar to that in panel B except that the cells used in panel C were assayed. Data in panels A and C are representative of two experiments with nearly identical results. Data in panels B and D are representative of three experiments with similar results. Error bars indicate SEM.