FIG 10.

Accumulation of mRNA in doubly labeled rcTLP-infected cells. (A) Atto 647N-labeled DLPs (red) colocalize with various numbers of Atto 565-labeled oligonucleotides (green) taken from images after 15, 30, and 60 min of incubation with doubly labeled rcTLPs. Particles of interest are arranged vertically as follows: top, 560-nm channel; middle, 640-nm channel; and bottom, overlay. The numbers of colocalized oligonucleotides increase from left to right, with the rightmost particle representing the maximum number of colocalized oligonucleotides from all images collected at the respective time point. The white, blue, and green arrows point to the particles of interest from a single image collected after 1 h of incubation. (B) z-projection of the region surrounding the 4 and 60 oligonucleotide-colocalized particles from the 1-h data in panel A. Red represents the labeled DLP as in panel A, and yellow represents a particle that colocalizes with Atto 488-labeled VP7 and so has yet to lose the VP7 shell. White and green arrows correspond to the particles of interest as in panel A. (C) z-projection of the 640-nm channel of a 1-h-infected cell. The representative 4, 30, and 60 oligonucleotide-colocalized DLPs were taken from this image, with their respective locations in the cell marked by colored boxes (white, green, and blue), corresponding to the arrows in panel A.