FIG 2.

Infectivity of RRV and fluorescently labeled rcTLPs. Focus-forming assays comparing native RRV (TLP) to the two differently labeled rcTLP preparations employed in this work were performed. For rcTLP-1, the DLPs and VP7 were labeled with Atto 647N and Atto 488 dyes, respectively. For rcTLP-2, the DLPs and VP7 were labeled with Atto 488 and Atto 390 dyes, respectively. Infectivity is shown in focus-forming units per milliliter from triplicate experiments in the BSC-1 (dark gray) and MA104 (light gray) cell lines. Standard deviations of the three measurements are shown as error bars. The specific infectivity (particle per focus-forming unit [P/FFU]) of the virus in each sample is shown above the respective bars along with its standard deviation.