TABLE 5.
Sequences of the 44 20-base oligonucleotides generated for the 11 RRV gene segments using the Stellaris RNA FISH probe designera
| Gene product (gene accession no.) | Probe sequences |
|---|---|
| VP1 (EU636924) | AGATTAGATTATACTTCCCC, TGAACTGCGGATTGTGAGTT, AGCTTTTTCAATGATAGTCC, GGCATTCTCTATAACATCGC |
| VP2 (EU636925) | TAAATTCGTCTCACGACGCG, CTTCTTTTTCTTGCATTCGA, TGAGATAGCACTTTCTCTGA, TATCTGTAATCACCTCTTCC |
| VP3 (EU636926) | ATGTTTACACCATTAGAGGT, CACACCGTGTCTTAAAGCTA, ATAGATTTGGGTGTCTGCAT, TGCATTCTCGTAACTGTCTT |
| VP4 (EU636927) | TCATCAGATAAGTCAACGGT, TTTGCGTTTTTGTAGATCCA, TGAGCAAAAGGACCTGGATT, AGTTAACTGGAGCATAACCT |
| NSP1 (EU636928) | AAAGGTTGCCATGGCTAACA, TTATTCGAAGAGACTGGAGT, CTAGCGAACATCCGTGACAA, TGGTTCATTGTCCAAGAAGC |
| VP6 (EU636929) | ACAAGGAGTACAGGACATCC, TGGAGTATAATGTGCCTTCG, CCAATTCCTCCAGTTTGAAA, TCCAATTTCTAATCGGCAGA |
| NSP3 (EU636930) | ACTCCATCTTGAGCATCAAC, AGAAGAAGCCATCTGCTGAG, ACTGCAGCTTCAAATGAAGA, CAAGAGTAGAAGTTGCAGCA |
| NSP2 (EU636931) | TACACCGCAAGGCTCAAACG, CAAAAGCAAGCTAGCTCAGC, CTATAGCTATCGTTCTCCAA, GGTGCGATACCATAAATTAT |
| VP7 (EU636932) | AAAAGGAGCTAACCGCTAGC, TGAGCGATATCAGAAAGGTT, GTCCATCATTCTAGTCAAAG, GGGCTTTTAGTAATGGTGAC |
| NSP4 (EU636933) | AAGCTTTTCCATCTTTCCGC, CTCAATGTGTAGTTGAGGTC, TATGGAAAATACGCCATTCC, GTACAGTTAGGACAGAAGCA |
| NSP5 (EU636934) | GTCACGTCAATACTGAGAGA, TGCTGGAGGAAATAGATGGA, CCAGAAAGAGTTGACGTCGT, GTTCACTCCTACCAATAGAT |
a
Biosearch Technologies, Inc., Petaluma, CA.