FIG 3.

Interaction of LP-40 and control peptides with an NHR-derived peptide. (A to C) The thermodynamic profiles of the molecular interactions between T20 (A), T20-TRM (B), or LP-40 (C) and N39 were determined using ITC technology. The titration traces are shown at the top, and the binding affinities when the N39 solution was injected into an T20, T20-TRM, or LP-40 solution are shown at the bottom. (D) Visualization of the binding of LP-40 and control peptides to the NHR peptide N39 or N36 by native PAGE; each of the peptides was used at a final concentration of 40 μM. (E) Dose-dependent binding of T20 and LP-40 (40 μM) to N39 (0, 20, and 40 μM).