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. 2017 Aug 24;91(18):e00391-17. doi: 10.1128/JVI.00391-17

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Copyright © 2017 Pillay et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — The identity of the AAV-BP is AAVR, the multiserotype AAV receptor. (A) Virus overlay assay of cell membrane fractions from different types of cells. Membrane proteins were extracted from various types of cells, and 100 μg of membrane proteins was used to perform a virus overlay assay with purified wild-type AAV2 particles (56). The arrow indicates a strong binding band at 150 kDa, designated AAV-BP. (B) Summary of the top five genes that correspond to the peptide sequences from the mass spectrometry analysis of the AAV-BP band. AAVR is also denoted KIAA0319L. (C) One hundred micrograms of PSA-purified HeLa S3 membrane proteins was separated on a 2-D gel and transferred onto a PVDF membrane for a virus overlay assay with rAAV2, followed by reprobing with anti-LDLR, anti-ApoER2, anti-ORP150, or anti-AAVR antibody. (D) One hundred micrograms of N- and O-deglycosylated crude HeLa S3 cell membrane proteins was separated by 2-D electrophoresis and underwent a virus overlay assay, followed by reprobing with a rabbit polyclonal antibody to integrin α5. Squares indicate an identical area of the membrane.