FIG 2.
AAV-BP is absent in AAVRKO cells, reiterating its identity as AAVR. Purified membrane proteins from wild-type (WT) and AAVRKO (KO) cells (derived from the respective cell lines) were solubilized in PB–2% DMT. Twenty micrograms of the solubilized membrane proteins was separated on an SDS–6% PAGE gel and transferred onto a PVDF membrane for a virus overlay assay with rAAV2 (A), followed by reprobing with anti-AAVR antibody (B). The arrow indicates a strong band at 150 kDa in both the virus overlay and the immunoblot.