FIG 6.

Binding of AAVR PKD2 in virus overlay assays requires full-length AAVR PKD2. (A and B) Schematic depicting GST-tagged AAVR ectodomain constructs comprising PKD2 and various PKD2 ectodomain mutants, created by removing C-terminal amino acids (A) or N-terminal amino acids (B). (C and D) Gel electrophoresis was carried out on bacterial lysates transformed with the respective constructs in panels A and B, and a virus overlay assay with AAV2 was performed, followed by reprobing with an anti-GST antibody for N-terminal deletion constructs (C) or C-terminal deletion constructs (D).