FIG 5.
Tg7 mice have various degrees of membrane polarization regardless of prion infection. Isolated brain mitochondria from Tg7Sc mice euthanized at clinical stages of prion disease (n = 6 mice in duplicate), Tg7NBH-inoculated, age-matched control mice (n = 4 mice in duplicate), or NBH-inoculated WT C57BL/6 mice (n = 4 mice in duplicate) were assayed for their mitochondrial membrane potential using the membrane potential-sensitive dye JC-1. For valinomycin-treated samples, each symbol represents an individual mouse. Mito, mitochondria; Val, valinomycin. Significance was determined using a one-way analysis of variance (ANOVA) with Bonferroni's multiple-comparison test and a 95% confidence threshold. Error bars represent standard deviations of the mean. NS, not significant. **, P ≤ 0.005; ****, P ≤ 0.0001.