FIG 4.

p65 translocation in rMuV-infected cells. A549 cells were infected with rMuV-SH or rMuV-SHstop at an MOI of 5. (A) At 20 h p.i., cells were fixed and stained for p65 using a specific Cy3-coupled antibody, the viral N protein was stained using a specific Alexa 488-coupled antibody, and the nuclei were DAPI stained (not shown). Images were generated using a Zeiss cLSM780 confocal laser scanning microscope. Representative syncytia are shown in a closeup. (B) A total of 1.6 × 10⁴ cells from three individual experiments were analyzed for p65 translocation in infected cells at 15 and 20 h p.i. The graph depicts means ± SD. ****, P < 0.0001; results were calculated by unpaired t tests with a two-tailed P value.