Skip to main content
. 2017 Aug 24;91(18):e00593-17. doi: 10.1128/JVI.00593-17

FIG 8.

FIG 8

Expression of pUL31 is sufficient to reduce rRNA levels. (A) Domains identified within full-length UL31, including nuclear localization sequence (NLS), arginine-rich motifs (RxnR) within an intrinsic disorder region (IDR), and a conserved domain in betaherpesviruses (DUF570) containing a predicted dUTPase. The truncated UL31224 contains only NLS, RxnR, and IDR motifs. (B) U373 cells were transfected with pUL31-HA, pUL29-HA, pUL76-HA, or pUL31234-HA expression vectors. Cells were fixed and permeabilized at 18 h posttransfection and evaluated with immunofluorescence analysis using an anti-HA antibody. (C) U373 cells were transfected with the indicated vectors, including an empty control (v). Pre-rRNA levels relative to those of GAPDH were determined from total RNA isolated at 18 h posttransfection using qRT-PCR. Data are from three biological and two technical replicates, with error bars representing standard deviations from the means. (D) U373 cells were transfected with empty vector or with pUL31-HA or pUL31234-HA expression vector, and at 18 h posttransfection they were infected using UL31YFP or UL31stop virus at an MOI of 3. Total RNA was isolated at 96 hpi, and pre-rRNA levels were determined using qRT-PCR and primers against the 5′ external transcribed spacer (ETS) relative to GAPDH. Data are from three biological and two technical replicates, with error bars representing standard deviations from the means.