Blocking COPII transport reduced HCV entry. (A and B) Huh7.5.1 cells treated with an siRNA targeting Sec24C or claudin-1 5′ UTR or with a control siRNA for 72 h were infected with genotype 1b HCVpp (Con1) or VSVpp for another 72 h, followed by measurement of the luciferase activity and cellular ATP levels. The relative luciferase activity was normalized to the cellular ATP level. (C) Huh7.5.1 cells treated with Sec24C siRNA or a control siRNA for 72 h were infected with Jc1 for another 24 h, followed by measurement of the luciferase activity and cellular ATP levels. The relative luciferase activity was normalized to the cellular ATP level. (D and E) Huh7.5.1 cells treated with different doses of XN for 12 h were infected with genotype 1b HCVpp (Con1) or VSVpp for 3 days, followed by measurement of the luciferase activity. For all panels, data are presented as the means ± SD. **, P < 0.01; ***, P < 0.001.