FIG 7.

HCV entry could be rescued by expression of wild-type Sec24C but not the cargo binding-deficient mutant. (A) Protein expression levels of wild-type or mutant (⁸⁹⁵LIL⁸⁹⁷ to ⁸⁹⁵AAA⁸⁹⁷) siRNA-resistant Sec24C were measured by Western blotting. Huh7.5.1 cells were treated with siRNA targeting the Sec24C or a control siRNA for 24 h and transfected with a construct expressing wild-type or mutant siRNA-resistant Sec24C for 48 h. (B and C) Then the cells were infected with genotype 1b HCVpp (Con1) for another 72 h (B) or infected with Jc1 for another 24 h (C). The infection activity and ATP levels were measured. The relative luciferase activity was normalized to the cellular ATP levels. Data are presented as the means ± SD. *, P < 0.1; ***, P < 0.001.