FIG 1.

Genetic map of HBoV1 (1, 5, 8, 11, 12, 60). The HBoV1 ssDNA genome is shown in the negative sense, along with its transcriptional and posttranscriptional units, including the P5 promoter, 5′ splice donor sites (D1, D1′, D2, and D3), 3′ splice acceptor sites (A1, A1′, A2, and A3), the proximal polyadenylation site [(pA)p], the distal polyadenylation site [(pA)d], and the 3′ noncoding region (NCR). The left-end hairpin (LEH) and right-end hairpin (REH) structures of the genome are shown. Six groups of major HBoV1 mRNA transcripts are shown with either long-form mRNA (RX_L) that reads through the (pA)p site or short-form mRNA (RX_S) that is polyadenylated at the (pA)p site. R1 mRNA has a minor species (R1m) that is unspliced at the central small intron (D3-A3) and encodes a minor protein, NS1-70. Major ORFs are depicted as colored boxes, with the nucleotides (nt) and amino acids (aa) at the start and stop codons indicated. Proteins expressed from each mRNA are indicated beside their respective mRNA transcripts (molecular mass in kilodaltons). A bocaviral noncoding small RNA (BocaSR) is transcribed from the 3′ noncoding region spanning nucleotides 5199 to 5338, as indicated.