TABLE 2.
Modulation of OMV production in EHEC O104:H4 and O157:H7 by antibiotics and mitomycin C determined by quantification of total OMV protein contents
| Antibiotic |
LB226692 (O104:H4) |
5791/99 (O157:H7) |
||
|---|---|---|---|---|
| Fold increasea | Pb | Fold increasea | Pb | |
| None | 1.0 ± 0.13 | 1.00 | 1.0 ± 0.16 | 1.00 |
| Ciprofloxacin | 298.22 ± 51.18 | <0.05 | 217.28 ± 42.53 | <0.05 |
| Fosfomycin | 83.13 ± 21.97 | <0.05 | 31.13 ± 6.04 | <0.05 |
| Meropenem | 32.25 ± 5.43 | <0.05 | 19.32 ± 5.12 | <0.05 |
| Polymyxin B | 12.17 ± 3.65 | <0.05 | 9.03 ± 1.88 | <0.05 |
| Gentamicin | 1.25 ± 0.31 | 0.40 | 1.24 ± 0.36 | 0.59 |
| Rifaximin | 1.16 ± 0.27 | 0.46 | 1.29 ± 0.58 | 0.60 |
| Tigecycline | 1.24 ± 0.32 | 0.40 | 1.23 ± 0.39 | 0.59 |
| Azithromycin | 1.23 ± 0.26 | 0.40 | 1.19 ± 0.52 | 0.63 |
| Chloramphenicol | 1.03 ± 0.12 | 0.71 | 1.12 ± 0.38 | 0.64 |
| Mitomycin C | 616.96 ± 86.27 | <0.05 | 536.89 ± 79.99 | <0.05 |
a
Fold increase of total OMV protein concentration in the presence of the indicated antibiotic compared to that in the absence of antibiotic. Means of data from three experiments ± standard deviations are shown.
b
P values for the differences between the total OMV protein concentrations in the presence of the indicated antibiotics compared to that in the absence of antibiotics (one-sample t test with P value adjustments for multiple comparisons by the Benjamini–Hochberg method); a P value of <0.05 was considered significant.