TABLE 1.
Resistance profiles of the parental K. pneumoniae Kp41, transconjugant TcKp41, and recipient E. coli C600 strains
| Antibiotic | MIC (μg/ml) for: |
||
|---|---|---|---|
| Kp41a | TcKp41 (pKp41 M)b | E. coli C600 | |
| Nalidixic acid | >528 | 16 | 2 |
| Ciprofloxacin | >16 | 0.25 | 0.03 |
| Enrofloxacin | >16 | 1 | 0.03 |
| Marbofloxacin | >16 | 0.5 | 0.03 |
| Cefotaxime | >128 | >128 | 0.125 |
| Ceftiofur | >128 | >128 | 0.125 |
| Ceftazidime | 128 | 2 | 0.25 |
| Cefepime | >128 | >128 | 0.125 |
| Amikacin | 8 | 32 | 4 |
| Gentamicin | >128 | 64 | 2 |
a
Kp41 presents mutations in gyrA (Ser83Ile) and parC (Ser80Ile) and harbors blaCTX-M-15 in an IncF-type plasmid (data not shown).
b
Transconjugants were selected on MacConkey agar containing streptomycin (2,000 μg/ml), cefotaxime (2 μg/ml), and ciprofloxacin (0.06 μg/ml).