TABLE 1.
dPCRgyrA results for the mutant-LPP mixturesa
| Mutant strain (mutation) | Mutant/LPP ratio | No. of copies/μl |
Mean % of mutants (SD) | |||||
|---|---|---|---|---|---|---|---|---|
| Wild type (VIC) |
Mutant (FAM) |
|||||||
| Chip 1 | Chip 2 | Mean (SD) | Chip 1 | Chip 2 | Mean (SD) | |||
| LPPI1 (T83I) | 1:1 | 660.31 | 482.67 | 571.49 (125.61) | 655.22 | 651.94 | 653.58 (2.32) | 53.63 (5.41) |
| 1:10 | 1,601.80 | 1,550.60 | 1,576.20 (36.20) | 119.84 | 129.20 | 124.52 (6.62) | 7.33 (0.52) | |
| 1:100 | 1,393.90 | 1,385.60 | 1,389.75 (5.87) | 9.88 | 6.87 | 8.38 (2.13) | 0.60 (0.15) | |
| 1:500 | 1,534.70 | 1,369.30 | 1,452.00 (116.96) | 4.83 | 2.31 | 3.57 (1.78) | 0.24 (0.10) | |
| 1:1,000 | 1,249.90 | 1,325.00 | 1,287.45 (53.10) | 0.83 | 1.27 | 1.05 (0.31) | 0.08 (0.02) | |
| LPPI4 (D87N) | 1:1 | 434.52 | 432.43 | 433.48 (1.48) | 365.80 | 353.98 | 359.89 (8.36) | 45.36 (0.49) |
| 1:10 | 1,075.90 | 1,091.20 | 1,083.55 (10.82) | 81.92 | 80.50 | 81.21 (1.00) | 6.97 (0.14) | |
| 1:100 | 1,353.00 | 1,303.70 | 1,328.35 (34.86) | 9.88 | 8.43 | 9.16 (1.03) | 0.68 (0.06) | |
| 1:500 | 1,412.70 | 1,450.50 | 1,431.60 (26.73) | 1.83 | 2.66 | 2.25 (0.59) | 0.16 (0.04) | |
| 1:1,000 | 1,462.10 | 1,418.80 | 1,440.45 (30.62) | 0.94 | 0.87 | 0.91 (0.05) | 0.06 (0.002) | |
| LPPI5 (D87H) | 1:1 | 633.68 | 650.10 | 641.89 (11.61) | 510.10 | 533.82 | 521.96 (16.77) | 44.84 (0.35) |
| 1:10 | 1,306.80 | 1,299.30 | 1,303.05 (5.30) | 98.79 | 95.77 | 97.28 (2.14) | 6.95 (0.12) | |
| 1:100 | 1,099.90 | 1,284.50 | 1,192.20 (130.53) | 7.89 | 8.45 | 8.17 (0.40) | 0.68 (0.04) | |
| 1:500 | 1,189.40 | 1,112.90 | 1,151.15 (54.09) | 2.30 | 1.82 | 2.06 (0.34) | 0.18 (0.02) | |
| 1:1,000 | 1,311.20 | 1,282.70 | 1,296.95 (20.15) | 1.33 | 0.87 | 1.10 (0.33) | 0.08 (0.02) | |
a
The results of each chip are presented, followed by the means and the standard deviations (SDs) from the duplicates.