FIG 6.

Diflunisal and ciprofloxacin show synergy in macrophages. Macrophages were infected with F. tularensis LVS bacteria for 2 h, followed by gentamicin treatment to kill extracellular bacteria, and cocultured with ciprofloxacin (0.25 μg/ml), diflunisal (8 μg/ml), or a combination of diflunisal (2 μg/ml) and ciprofloxacin (0.06 μg/ml) for 22 h. Data shown are CFU counts of intracellular F. tularensis LVS in macrophages. ***, P < 0.001, comparing results with diflunisal to those with the DMSO control at the 24-h time point using two-way ANOVA with a Bonferroni posttest correction.