FIG 8.

Recovery of Csu pilus and biofilm repression with THF but not folate supplements under Tmp-mediated folate stress conditions. (A) CsuA/B expression with folate (Fol) or THF supplements, as determined by Western blotting with the anti-CsuA/B antibody (green bands); RNAP was used as loading control (red bands). Folate stress was generated by Tmp. S/T, Smx and Tmp; Kan, kanamycin. (B) Representative crystal violet assay images of A. baumannii strains with folate or THF supplements. THF but not folate was able to relieve folate stress generated by Tmp, resulting in partially restored biofilm formation. (C) CFU counts (top) and biofilm crystal violet assay quantification (bottom). Points indicate mean values, and error bars indicate standard deviations of triplicate samples. *, P ≤ 0.005; †, P ≤ 0.05, significant reduction of biofilm production, compared to 17978pAB3− cells with folate.