TABLE 3.
pncA mutation profilea
| Nucleotide change | Amino acid change(s) | SNP location(s) | No. of cases | M. tuberculosis lineage(s)b | MGIT PZA susceptibility assay resultb,c |
|---|---|---|---|---|---|
| Single mutation | |||||
| (−4) ins Cd | NA | Promoter | 1 | EAI5 | S |
| 149 ins Td | Frameshift at Cd50 | 149 | 1 | Beijing | S |
| 156 ins Td | Frameshift at Cd53 | 156 | 1 | Beijing | R |
| 159 ins Ad | Frameshift at Cd54 | 159 | 1 | T1 | S |
| 166 ins Gd | Frameshift at Cd56 | 166 | 1 | Beijing | S |
| 391 ins GG | Frameshift at Cd131 | 391 | 1 | Beijing | R |
| 392 ins GG | Frameshift at Cd131 | 392 | 1 | EAI1-SOM | R |
| 445 ins GCd | Frameshift at Cd149 | 445 | 6 | LAM9 (6) | R (6) |
| 450 ins GCd | Frameshift at Cd150 | 450 | 1 | Beijing | R |
| 518 ins Gd | Frameshift at Cd173 | 518 | 1 | Beijing | R |
| del 558 Gd | Frameshift at Cd186 | 558 | 1 | EAI | R |
| A(−11)G | NA | Promoter | 2 | Beijing (1), CAS1-Delhi (1) | R, R |
| A(−15)C | NA | Promoter | 3 | T1 (2), Orphan (1) | S, S, R |
| AAG to ATG | Lys96Met | 287 | 1 | LAM9 | R |
| AAT to AGTd | Asn149Ser | 446 | 1 | T1 | S |
| ACC to CCC | Thr47Pro | 139 | 2 | Beijing | R (2) |
| ACT to CCT | Thr76Pro | 226 | 1 | EAI1-SOM | R |
| AGC to AGG | Ser104Arg | 312 | 2 | Beijing (2) | R, S |
| ATT to ACT | Ile133Thr | 398 | 1 | Orphan | R |
| CAA to TAA | Gln122Stop | 364 | 1 | Beijing | S |
| CAC to CAG | His51Gln | 153 | 1 | T1 | R |
| CAC to CCC | His51Pro | 152 | 1 | Beijing | R |
| CAG to AAG | Gln10Lys | 28 | 1 | LAM10-CAM | R |
| CAG to TAG | Gln10Stop | 28 | 1 | EAI1-SOM | R |
| CAT to CCT | His137Pro | 410 | 1 | T1 | R |
| CAT to CGT | His71Arg | 212 | 1 | Beijing | R |
| CAT to CTTd | His137Leu | 410 | 1 | T1 | R |
| CCG to TCG | Pro54Ser | 160 | 1 | Orphan | S |
| CCG to CTG | Pro54Leu | 160 | 1 | Orphan | R |
| CTG to CCG | Leu172Pro | 515 | 1 | T1 | R |
| CGG to TGGd | Arg2Trp | 4 | 1 | LAM9 | S |
| del 340 Ad | Frameshift at Cd114 | 340 | 1 | Beijing | R |
| del 112 to 561d | del of Cd37 to Cd187 | 112–561 | 3 | Beijing (3) | R (3) |
| del 296 to 309d | Frameshift at Cd99 | 296–309 | 1 | T1 | R |
| GAC to AAC | Asp49Asn | 145 | 1 | Beijing | R |
| GAC to GCC | Asp12Ala | 35 | 1 | LAM9 | R |
| GCC to ACCd | Ala28Thr | 82 | 1 | EAI3-IND | S |
| GCG to ACG | Ala171Thr | 511 | 1 | Beijing | R |
| GCG to GTG | Ala171Val | 512 | 1 | T1 | S |
| GCG to GGGd | Ala171Gly | 512 | 1 | T1 | S |
| GGC to GACd | Gly105Asp | 314 | 3 | T1 (3) | R (2), S (1) |
| GGC to GAC | Gly24Asp | 71 | 1 | Beijing | R |
| GTG to GCG | Val139Ala | 416 | 1 | T1 | R |
| GTG to ATGd | Val155Met | 463 | 1 | EAI-SOM | R |
| TAC to CAC | Tyr103His | 307 | 1 | T1 | S |
| TAC to TAA | Tyr95Stop | 285 | 1 | T1 | R |
| TAC to TAG | Tyr99Stop | 297 | 3 | Beijing | R (2), S (1) |
| TAC to TGC | Tyr103Arg | 308 | 1 | Orphan | R |
| TCC to TCT | Ser65Ser | 195 | 10 | CAS1-Delhi (10) | S (8), R (2) |
| TGT to GGTd | Cys138Gly | 412 | 1 | Orphan | R |
| TTC to GTCd | Phe58Val | 172 | 1 | Beijing | S |
| TTG to TGG | Leu4Trp | 12 | 2 | CAS1-Delhi (2) | R (2) |
| Multiple mutations | |||||
| TCC to TCT, 295 ins C,d 430 ins C,d 467 ins Cd | Ser65Ser and frameshift at Cd99,144,156 | 195, 295, 430, 467 | 1 | CAS1-Delhi | S |
| AAG to GAG, AGG to ACG, GAG to CAGd | Lys96Glu, Arg154Thr, Glu181Gln | 286, 461, 541 | 1 | EAI5 | R |
| TCC to TCT, ACT to ATT | Ser65Ser, Thr76Ile | 195, 227 | 1 | CAS2 | R |
| TGG to TGA, 445 ins GC | Trp119Stop, frameshift at Cd149 | 357, 445 | 1 | LAM9 | R |
| GAC to GCC, 178 ins Cd | Asp12Ala, frameshift at Cd60 | 35, 178 | 1 | LAM9 | S |
| A(−15)C, TCG to CCG | NA, Ser67Pro | Promoter, 199 | 1 | T1 | R |
| GCG to GGG,d GGC to GAC | Ala38Gly, Gly105Asp | 113, 314 | 1 | T1 | R |
| TCC to TCT, GGT to AGT | Ser65Ser, Gly97Ser | 195, 289 | 1 | CAS1-Delhi | R |
| TAC to TGC, TGG to CGG | Tyr34Cys, Trp68Arg | 101, 202 | 1 | LAM9 | S |
a
SNP, single nucleotide polymorphism; R, resistant; S, sensitive/susceptible; ins, insertion; del, deletion; NA, not applicable; nt, nucleotide; Cd, codon.
b
Numbers in parentheses indicate the number of isolates of that type.
c
Results of the MGIT assay for PZA susceptibility separated by a comma indicate the corresponding result for the M. tuberculosis lineage.
d
New mutation.